NAHAS, E., AUFY, S., ISMAIL, M., MOHAMED, R., EL AKKAD, D. (2022). VALUE OF MICROSCOPY AND COPROANTIGEN FOR DETECTION OF CRYPTOSPORIDIUM AND GIARDIA IN STOOL SAMPLES. test, 52(2), 261-266. doi: 10.21608/jesp.2022.257454
ENGY AHMED ABDALLAH NAHAS; SAYEDA M. AUFY; MOUSA A. M. ISMAIL; REHAM KAMAL MOHAMED; DINA M. H. EL AKKAD. "VALUE OF MICROSCOPY AND COPROANTIGEN FOR DETECTION OF CRYPTOSPORIDIUM AND GIARDIA IN STOOL SAMPLES". test, 52, 2, 2022, 261-266. doi: 10.21608/jesp.2022.257454
NAHAS, E., AUFY, S., ISMAIL, M., MOHAMED, R., EL AKKAD, D. (2022). 'VALUE OF MICROSCOPY AND COPROANTIGEN FOR DETECTION OF CRYPTOSPORIDIUM AND GIARDIA IN STOOL SAMPLES', test, 52(2), pp. 261-266. doi: 10.21608/jesp.2022.257454
NAHAS, E., AUFY, S., ISMAIL, M., MOHAMED, R., EL AKKAD, D. VALUE OF MICROSCOPY AND COPROANTIGEN FOR DETECTION OF CRYPTOSPORIDIUM AND GIARDIA IN STOOL SAMPLES. test, 2022; 52(2): 261-266. doi: 10.21608/jesp.2022.257454

VALUE OF MICROSCOPY AND COPROANTIGEN FOR DETECTION OF CRYPTOSPORIDIUM AND GIARDIA IN STOOL SAMPLES

Journal of the Egyptian Society of Parasitology
Volume 52, Issue 2, August 2022, Pages 261-266    PDF (3.42 M)
DOI: 10.21608/jesp.2022.257454
Authors
ENGY AHMED ABDALLAH NAHAS1; SAYEDA M. AUFY2; MOUSA A. M. ISMAIL2; REHAM KAMAL MOHAMED2; DINA M. H. EL AKKAD2
1Department of Parasitology, Faculty of Medicine, 6th October University
2Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt
Abstract
Giardiasis and cryptosporidiosis are common protozoa causing gastrointestinal troubles with symptoms
ranged from asymptomatic to long-lasting diarrhea with mal-absorption. The present work was
carried on a total of 96 cases; 62 of them presented with GIT symptoms with or without diarrhea, 50%
of them was previously diagnosed as giardiasis or cryptosporidiosis infected and treated 1-2 months
ago, 11 cases were free from GIT symptoms and 23 cases suffered from chronic debilitating disease as
cancer or renal disease, some of them have previous history of gastro-intestinal disturbances.
The rapid immunochromatographic test (ICT) and copro-ELISA detected G. lamblia and C. parvum
antigens in all fecal samples compared to formol ether concentration and Modified Ziehl-Neelsen
(MZN) staining technique. The sensitivity of G. lamblia in stool samples was 60%, 98.3% & 96.6%
using concentration technique, ICT & ELISA respectively, and specificity was 98.75%, 96.43% &
96.43% respectively. The sensitivity of C. parvum with MZN stain and antigen detection by ICT&
ELISA was 46.2%, 96.4% & 100% respectively. Specificity of the tests in detecting C. parvum was
better than Giardia; 96.4% -100% respectively. All tests have 100% PPV and 80-888% NPV for the
immunological techniques with superiority of ICT over ELISA.
Keywords
Giardia; Cryptosporidium; ELISA; Coproantigen; ICT; MZN; formol ether concentration
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