Biochemical studies on some polysaccharides and their effects on diabetic rats | ||
| Menoufia Journal of Agricultural Biotechnology | ||
| Article 2, Volume 7, Issue 3, October 2022, Pages 49-50 PDF (132.91 K) | ||
| Document Type: original papers | ||
| DOI: 10.21608/mjab.2022.268990 | ||
| Authors | ||
| Y. A. Ashoush1; A. M. F. Ali1; M. M. Abozid1; M. M. S. Asker2 | ||
| 1Prof. of Biochemistry, Fac. of Agric., Menoufia Univ. | ||
| 2Prof. of Microbial Biotechnology, National Research Center | ||
| Abstract | ||
| This study aimed to evaluate the effect of polysaccharide extract of okra, aloe vera and purslane concentration of (100-200 mg / kg of bw) on diabetic rats after injecting them with alloxan concentration of 150 mg/ kg. The chemical composition of okra, aloe vera and Purslane were found that Okra contains 87.49% carbohydrates, 72.55% total soluble polysaccharides, 21% extraction percentage, while aloe vera contains 90.22% carbohydrates, 81.74% total soluble polysaccharides, 32% extraction rate, while purslane contains 79.51% carbohydrates, 63.0% total soluble polysaccharides, 17.5% extraction percentage. While the infrared analysis proved in the spectral range the presence of OH, CH2, C=O, COO-, S=O, C-O-C, C-O-H and C-O groups, as well as alpha-pyranose rings, and alpha and beta glycosidic bonds for the okra, aloe vera and purslane. An estimated of the molecular weight of the polysaccharides of okra was 5.29 x 10 4 g/mol, aloe vera 6.98 x 10 4 g/mol, and purslane 1.25 x 10 4 g/mol. HPLC analysis of polysaccharides was found which okra contains manuronic acid 0.52, glucose 1.22, mannose 0.96, fucose 0.35 and arabinose 1.0 molar, and aloe vera contains manuronic acid 0.29, glucose 0.35, fructose 1.26, galactose 2.64, and arabinose 1.0 molar, while purslane contains manuronic acid 0.36, glucose 1.51, galactose 1.0 and arabinose 1.6 molar. The study showed that the polysaccharide extract of okra, aloe vera and purslane grown in Egypt was very beneficial in improving the glucose level in the plasma of diabetic rats. And leads to an improvement in the results of glycated hemoglobin. It also improved kidney function (urea - creatinine) and liver functions (GOT - GPT – ALP) and it had a qualitative effect on improving the synthetic functions of the liver (total protein - albumin). Also leads to an improvement in the level of results of the advanced glycated end products. The biological experiment indicated that the results of all groups treated with polysaccharide extract of okra, aloe vera and purslane at a concentration of 100-200 mg/kg improved, and that the best treated groups at all were those treated with polysaccharide extract of okra and aloe vera concentration of 200 mg/kg after 45 days, and this improvement may be due a high molecular weight of okra and aloe vera, or to the presence of some rare monosaccharides such as fucose and mannose in okra, galactose and fructose in aloe vera and high mannuronic acid in plants. | ||
| Keywords | ||
| Polysaccharides; okra; purslane; monosaccharides; molecular weight; HPLC | ||
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