Abdel maksoud elfaramawy, D., H. Abo ghalia, H., M. Ashour, S., A. Mohamed, A., S. Zaki, S. (2022). Evaluation of the Native Killer Yeasts against the Postharvest Phytopathogenic mould of Balady Orange Fruits. test, 39(2), 23-48. doi: 10.21608/jsrs.2022.275786
Doaa Saber Abdel maksoud elfaramawy; Hoda H. Abo ghalia; Sanaa M. Ashour; Ahmed A. Mohamed; Sanaa S. Zaki. "Evaluation of the Native Killer Yeasts against the Postharvest Phytopathogenic mould of Balady Orange Fruits". test, 39, 2, 2022, 23-48. doi: 10.21608/jsrs.2022.275786
Abdel maksoud elfaramawy, D., H. Abo ghalia, H., M. Ashour, S., A. Mohamed, A., S. Zaki, S. (2022). 'Evaluation of the Native Killer Yeasts against the Postharvest Phytopathogenic mould of Balady Orange Fruits', test, 39(2), pp. 23-48. doi: 10.21608/jsrs.2022.275786
Abdel maksoud elfaramawy, D., H. Abo ghalia, H., M. Ashour, S., A. Mohamed, A., S. Zaki, S. Evaluation of the Native Killer Yeasts against the Postharvest Phytopathogenic mould of Balady Orange Fruits. test, 2022; 39(2): 23-48. doi: 10.21608/jsrs.2022.275786

Evaluation of the Native Killer Yeasts against the Postharvest Phytopathogenic mould of Balady Orange Fruits

Journal of Scientific Research in Science
Article 3, Volume 39, Issue 2, December 2022, Pages 23-48    PDF (1.39 M)
Document Type: Original Article
DOI: 10.21608/jsrs.2022.275786
Authors
Doaa Saber Abdel maksoud elfaramawy* 1; Hoda H. Abo ghalia2; Sanaa M. Ashour2; Ahmed A. Mohamed3; Sanaa S. Zaki2
1Botany- faculty of women- ain shams university
2Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
3Plant Protection Research Institute, Agriculture Research Centre, Cairo, Egypt.
Abstract
Yeasts are some of the most important postharvest biocontrol agents (BCAs). Postharvest oranges frequently deteriorate due to green and blue moulds, leading to significant economic losses. The purposes of the present study were to isolate blue and green moulds from infected orange fruits, to assess the ability of killer yeasts isolated from healthy orange fruits and leaves from orange orchards to control blue and green moulds and to evaluate the additive effect of BCAs in combination with 2% sodium bicarbonate (SBC), 2%, sodium benzoate (SB), 2% calcium chloride, 0.2% salicylic acid (SA) or 0.5% chitosan. Among eight fungi isolated from orange fruits showing symptoms of green and blue mulds infection, two were identified as P. digitatum and P. italicum and selected for in vitro assays. Twenty eight yeast isolates were obtained from orange leaves and from the surface of fruits. All yeasts exhibited high killer activity. Twelve yeasts reduced 22.5 –70% of P. digitatum growth while seven isolates reduced 21.1- 68.5% of P. italicum growth. The most potent yeast isolates were identified as Candida pseudotropicalis, Candida salmanticensis, Candida membranifaciens and Pichia guilliermondii.  Combination of the BCAs, C. pseudotropicalis, C. salmanticensis and P. guilliermondii with SBC, CaCl2 or chitosan increased their effectiveness against P. digitatum. While combination of C. pseudotropicalis, C. membranifaciens and P. guilliermondii with these natural compounds decreased their effectiveness against P. italicum.  Combination of C. membranifaciens with SA increased its effectiveness against P. digitatum. Sodium benzoate has additive effect on C. pseudotropicalis against P. digitatum and C. pseudotropicalis and P. guilliermondii against P. italicum.
Keywords
Killer yeasts; Biocontrol agents; Phytopathogens; Orange fruits
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