Ramadan, M., Solyman, S., Yones, M., Halaby, H., Abdalla, Y., Hanora, A. (2019). Shotgun Metagenomic analysis of cutaneous microbiome in patients with atopic dermatitis. test, 3(1), 1-3. doi: 10.21608/rpbs.2019.7002.1020
Mohammed Ramadan; Samar Solyman; Mamdouh Yones; Hamada Halaby; Yaser Abdalla; Amr Hanora. "Shotgun Metagenomic analysis of cutaneous microbiome in patients with atopic dermatitis". test, 3, 1, 2019, 1-3. doi: 10.21608/rpbs.2019.7002.1020
Ramadan, M., Solyman, S., Yones, M., Halaby, H., Abdalla, Y., Hanora, A. (2019). 'Shotgun Metagenomic analysis of cutaneous microbiome in patients with atopic dermatitis', test, 3(1), pp. 1-3. doi: 10.21608/rpbs.2019.7002.1020
Ramadan, M., Solyman, S., Yones, M., Halaby, H., Abdalla, Y., Hanora, A. Shotgun Metagenomic analysis of cutaneous microbiome in patients with atopic dermatitis. test, 2019; 3(1): 1-3. doi: 10.21608/rpbs.2019.7002.1020

Shotgun Metagenomic analysis of cutaneous microbiome in patients with atopic dermatitis

Records of Pharmaceutical and Biomedical Sciences
Article 1, Volume 3, Issue 1, March 2019, Pages 1-3    PDF (231.51 K)
Document Type: Short communications
DOI: 10.21608/rpbs.2019.7002.1020
Authors
Mohammed Ramadan* 1; Samar Solyman2; Mamdouh Yones1; Hamada Halaby1; Yaser Abdalla3; Amr Hanora2
1Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Assiut, Egypt.
2Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University. Ismaillia, Egypt.
3Department of Dermatology, Faculty of Medicine, Al-Azhar University, Assiut, Egypt.
Abstract
Atopic dermatitis (AD) is a chronic complex inflammatory skin disorder. AD characterized by multiple contributing factors, such as impairment in skin barrier integrity, participation of either innate or adaptive immunity arms, in addition to the pivotal roles of cutaneous microbiome. The analysis of cutaneous microbiota was performed using shotgun metagenomic sequencing on an Illumina HiSeq platform in parallel with 16s rRNA genes using MiSeq sequencing. Two samples for each sequencing technique were collected from one child and one adult subjects with AD. Metagenomic data from 16srRNA was in accordance with those from shotgun sequencing. Taxonomic profile of samples at genus level showed overrepresentation of Streptococcus in child and Staphylococcus in adults. Species level analysis of reads revealed monoclonality of Staphylococcus aureus community in comparison to heterogeneity of Staphylococcus epidermidis. 16S rRNA gene based analysis was unable to provide comprehensive description of bacterial communities, while shotgun metagenomic analyses described the composition of microbiota with high resolution at different taxonomic levels especially subspecies and single nucleotide variants levels. Finally, further studies using shotgun metagenomic approaches will be required for characterization of microbiome in healthy and diseased subjects.
Keywords
atopic dermatitis; Shotgun metagenomic analysis; Microbiome; 16srRNA
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