Fahmy, D., Sidkey, N., Elkawaga, M., Zaid, D. (2016). ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC ACTINOMYCETES ISOLATED FROM LEAVES OF ASPHODELUS TENUIFOLIUS (CAV.) (MARSA MATROUH, EGYPT). test, 66(2), 373-386. doi: 10.21608/ejdr.2016.29639
Dina M. Fahmy; Nagwa Sidkey; Mai Elkawaga; Doaa S. Zaid. "ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC ACTINOMYCETES ISOLATED FROM LEAVES OF ASPHODELUS TENUIFOLIUS (CAV.) (MARSA MATROUH, EGYPT)". test, 66, 2, 2016, 373-386. doi: 10.21608/ejdr.2016.29639
Fahmy, D., Sidkey, N., Elkawaga, M., Zaid, D. (2016). 'ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC ACTINOMYCETES ISOLATED FROM LEAVES OF ASPHODELUS TENUIFOLIUS (CAV.) (MARSA MATROUH, EGYPT)', test, 66(2), pp. 373-386. doi: 10.21608/ejdr.2016.29639
Fahmy, D., Sidkey, N., Elkawaga, M., Zaid, D. ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC ACTINOMYCETES ISOLATED FROM LEAVES OF ASPHODELUS TENUIFOLIUS (CAV.) (MARSA MATROUH, EGYPT). test, 2016; 66(2): 373-386. doi: 10.21608/ejdr.2016.29639

ANTIMICROBIAL AND ANTIOXIDANT POTENTIALS OF ENDOPHYTIC ACTINOMYCETES ISOLATED FROM LEAVES OF ASPHODELUS TENUIFOLIUS (CAV.) (MARSA MATROUH, EGYPT)

Egyptian Journal of Desert Research
Article 7, Volume 66, Issue 2, December 2016, Pages 373-386    PDF (750.71 K)
Document Type: Original Article
DOI: 10.21608/ejdr.2016.29639
Authors
Dina M. Fahmy* 1; Nagwa Sidkey2; Mai Elkawaga2; Doaa S. Zaid2
1Natural Products Unit, Department of Medicinal and Aromatic Plants, Desert Research Center, El-Matareya, Cairo, Egypt
2Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, Egypt
Abstract
The study aimed to evaluate the antimicrobial and antioxidant abilities of four endophytic actinomycetes (strain Act1, Act2, Act3, and Act4) isolated from leaves of Asphodelus tenuifolius, as well as assessment of their phytochemical profile. Referring to morphological characters and 16S rDNA sequence analysis, actinomycete (strain Act2) was identified as Streptomyces fenghuangensis (GIMN4.003T) with 96% identical sequence and accession number (NR1175021). The results showed variant inhibitory activity against pathogenic bacteria and fungi. Act2endophytic actinomycete isolate showed broad spectrum activity against all pathogenic tested organisms, recording notable MIC value 10 µg/ml against Bacillus subtilus andBacillus cereus, while At3and At4showed strong impact on Candida albicans. All of the ethyl acatate extracts of actinomycete isolates revealed high antioxidant activity ranging from 45-82% using DPPH radical. Phytochemical screening of the isolates indicates the presence of phenolics, flavonoids, sterols, anthraquinones and naphthaquinones.
Keywords
Asphodelus tenuifolius; Antimicrobial; antioxidant; Streptomyces fenghuangensis; Phytochemical screening
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