Ibrahim, H., Hassan, M., Amin, R., Eleiwa, N., Nadim, S. (2015). Prevalence of E. coli and detection of virulent genes by multiplex PCR in meat products. test, 29(2), 268-273. doi: 10.21608/bvmj.2015.31735
Hemmat M. Ibrahim; Mohamed A. Hassan; Reham A. Amin; Nesreen Z. Eleiwa; Samaa S. Nadim. "Prevalence of E. coli and detection of virulent genes by multiplex PCR in meat products". test, 29, 2, 2015, 268-273. doi: 10.21608/bvmj.2015.31735
Ibrahim, H., Hassan, M., Amin, R., Eleiwa, N., Nadim, S. (2015). 'Prevalence of E. coli and detection of virulent genes by multiplex PCR in meat products', test, 29(2), pp. 268-273. doi: 10.21608/bvmj.2015.31735
Ibrahim, H., Hassan, M., Amin, R., Eleiwa, N., Nadim, S. Prevalence of E. coli and detection of virulent genes by multiplex PCR in meat products. test, 2015; 29(2): 268-273. doi: 10.21608/bvmj.2015.31735

Prevalence of E. coli and detection of virulent genes by multiplex PCR in meat products

Benha Veterinary Medical Journal
Article 33, Volume 29, Issue 2, December 2015, Pages 268-273    PDF (431.18 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.31735
Authors
Hemmat M. Ibrahim1; Mohamed A. Hassan1; Reham A. Amin1; Nesreen Z. Eleiwa2; Samaa S. Nadim2
1Department of Food Hygiene, Faculty of Veterinary Medicine, Benha University
2Meat hygiene, animal health research institute Tanta Branch
Abstract
A total of 100 meat products samples of minced meat, kofta, beef burger and sausage (25 samples of
each), weight of each sample10gm were collected from different shops and supermarkets in Cairo
governorate, to be investigated for the presence of E. coli and detection of virulent genes by modern
technique (PCR). The obtained results indicated that the incidence of E. coli isolated from the examined
samples were (4)16%, (7)28%, (6)24% and (11)44% of minced meat, kofta, beef burger and sausage,
respectively. Actually E. coli strains isolated from 18 positive E.coli were 9 strains O26, O55:H7, O103,
O111:H4, O114:H21, O119:H6, O124, O125:H21 and O128:H2. These isolated strains were investigated by using Multiplex PCR to detect presence of virulent genes (stx1, stx2 and eaeA) in each isolated strain. The
results obtained reported that E. coli O26 & O111 posses (3) genes stx1, stx2 and eaeA genes, E.coli O103,
O119 & O128 carry (2) genes stx1and stx2, E. coli O55 carry (2) genes stx1 and eaeA genes, E.coli O125
carry (2) genes stx2 and eaeA genes, E. coli O114 posses (1) gene stx1gene. While virulence genes were
not detected in E. coli O124.
Keywords
E.coli; meat products; PCR
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