Genetic Characterization and in Vitro Conservation of Quince and Hawthorn Grown at Saint Catherine Valleys | ||
| Alexandria Journal of Agricultural Sciences | ||
| Contributors, Volume 68, Issue 3, September 2023, Pages 230-235 PDF (1.46 M) | ||
| DOI: 10.21608/alexja.2023.325852 | ||
| Authors | ||
| Mohamed Salaheldin Mokhtar* 1; Mohammed M. Abdelfatah Yacout1; Hemaid Ibrahim Soliman2; Moustafa Mahmoud Eldakak1 | ||
| 1Genetics Department of Genetics Faculty of Agriculture University of Alexandria | ||
| 2Biotechnology Plant Tissue Culture Unit Genetic Resources Department Desert Research Center. | ||
| Abstract | ||
| The aim of this work is to study the factors affecting the in vitro propagation and developing of laboratory system to obtain a higher mass production of such important plants of the Egyptian desert. This study conducted on Quince (Cydonia oblonga Mill.) and Hawthorn (Crataegus sinaica Boiss.) grown at Saint Catherin valleys and mountains in the period from March 2017 to March 2019. The laboratory system of in vitro propagation of Quince (Cydonia oblonga Mill.) and Hawthorn (Crataegus sinaica Boiss.) was achieved by obtaining the best sterilization treatment for both plants and achieving a successful establishment by obtaining sterile cultures and increasing proliferation rate for both plants, in addition to increase multiply shoots in the jars by the best concentrations of PGRs in the multiplication stage. The rooting and acclimatization stage successfully achieved in Quince plantlets but did not achieve in Hawthorn plant, so, more studies were recommended in the future to achieve rooting stage in Hawthorn. And the conservation strategy for both plants by encapsulation/dehydration technique was done. The genetic characterization between collected individuals of both plants was performed using ISSR-PCR and the genetic distance between Tynia Valley Quince and Mousa Mountain Quince is shorter than the Jbaal Valley Quince, while the genetic distance between Jbaal Valley Hawthorn and Tynia Valley Hawthorn seems not existed and the plants collected from these regions were much similar to each other according to the genetic characterization but Mousa Mountain Hawthorn was more different from the other regions. In addition, the evaluation of gene stability was done also by using RAPD-PCR of in vitro propagated plants produced by tissue culture techniques. The resulted data revealed that there is not any differentiation in the genetic material of these plants. | ||
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