Resuscitation Using Hypertonic Saline, Effect on Muscle Performance and Wound Healing in A Rat Model of Burn | ||
| Egyptian Journal of Chemistry | ||
| Volume 67, Issue 7, July 2024, Pages 421-434 PDF (3.25 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2024.234184.8558 | ||
| Authors | ||
| Inas Harb1; Afaf Osman* 2; Abeer Mostafa3; Zeinab Hegab4; Marwa Mohamed Sabry4; Sara Kalifa5; Amr Maher2 | ||
| 1Department of Medical Pharmacology, Faculty of Medicine, Cairo University, Egypt | ||
| 2Department of Medical Pharmacology, Faculty of Medicine, Cairo University | ||
| 3Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University | ||
| 4Department of Histology, Faculty of Medicine, Cairo University | ||
| 5Department of Pathology, Faculty of Medicine, Cairo University | ||
| Abstract | ||
| Muscle wasting is a serious burn complication. The possible protective impact of hypertonic saline vs. ringer lactate on muscle wasting and wound healing in a rat burn model was investigated. Rats were divided into; control rats received normal saline, untreated burn rats, ringer lactate treated burn rats & hypertonic saline treated burn rats received normal saline, ringer lactate or hypertonic saline, respectively after induction of burn. After 18 hours, serum levels of substance P, tumor necrosis factor- alpha (TNF-α), interleukin-1beta (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), and interleukin-10 (IL-10) were measured. Wound healing was assessed after 2, 4, and 6 weeks. At the end of the 6th week, gastrocnemius muscle weight and performance, transforming growth factor (TGF-β), collagen type I, forkhead box transcription factors O (FOXO) gene expression and histopathological examination were done. Hypertonic saline treatment significantly lowered substance P, TNF-α, IL-1β, IL-6, IL-2 with elevated IL 10, improved muscle weight and performance, improved wound healing, reduced TGF ß, collagen I and FOXO genes compared with ringer lactate treatment. Histopathological findings supported these results. Early use of hypertonic saline in burn showed a more promising effect on the improvement of muscle performance and skin regeneration than ringer lactate solution does. | ||
| Keywords | ||
| Hypertonic saline; ringer lactate; muscle performance; wound healing; burn | ||
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