Ameliorating the toxic effect of the immunosuppressive drugs (Tacrolimus) on male Albino rat tongue by mesenchymal stem cells versus platelet rich plasma (histological, immunohistochemical and scanning electron microscopic study | ||
| Benha Medical Journal | ||
| Article 1, Volume 41, Issue 6, October 2024, Pages 1-16 PDF (1.61 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bmfj.2024.175374.1711 | ||
| Authors | ||
| Amal Elshazly* 1; Neama Mahmoud Taha2; Asmaa Hussein3; Naglaa Sarg4 | ||
| 1Department of anatomy and embryology benha faculty of medicine benha university | ||
| 2physiology department | ||
| 3Forensic and toxicology department | ||
| 4Department of anatomy Benha univerisity | ||
| Abstract | ||
| Abstract: Background: Toxic effects on the tongue caused by Tacrolimus remains a major problem. Therefore, the aim of this research was to evaluate the potential therapeutic bone marrow-derived mesenchymal stem cells (MSCs) and platelet rich plasma (PRP) impact on tongue of albino rat. Materials and Methods: 40 male Albino rats were divided into four equal groups. Group I control group received no treatment. Group II received daily subcutaneous injections of 1 mg/kg/day of Tacrolimus for 30 days. Group III received Tacrolimus for 30 days then a single PRP injection. Group IV received Tacrolimus for 30 days then a single injection of MSCs. In all groups the rats were sacarified after sixty days from the beginning of the experiment. For demonstration of the collagen fibers, tongue sections were stained with Mallory Trichrome and were stained with Hematoxylin and Eosin for histological analysis. Electron microscopic scan were used in this study and immunohistochemistry using anti- PCNA primary antibody were used to examine the tongue. Results: Histological and immunohistochemical examination of Tacrolimus group tongues showed poorly defined filiform papillae , some epithelial cells appeared degenerated with pyknotic nuclei, however tongue sections of (Gr III) and (Gr IV) showed marked tongue histological structure improvement and up regulated expression of PCNA, compared to the (Gr II). Scanning electron microscope supported these results. Conclusion: MSCs and PRP have good effects in reduction of the toxic effect of Tacrolimus administration on the rat tongue with insignificant difference between the two methods. | ||
| Keywords | ||
| Keywords: Tacrolimus; MScs; PRP; tongue toxicity | ||
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