APPLICATION OF EMBRYO TRANSFER IN FRIESIAN COWS UNDER EGYPTAN CONDITIONS | ||
| Egyptian Journal of Agricultural Research | ||
| Article 35, Volume 77, Issue 3, September 1999, Pages 1415-1431 PDF (4.52 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.1999.337765 | ||
| Authors | ||
| KAMEL M. E. MOHAMMED1; SAYED T. ISMAIL2 | ||
| 1Animal Reproduction Research Institute. Agricultural Research Centre, Dokki, Giza, Egypt | ||
| 2Faculty of Veterinary Medicine, Cairo University, Giza, Egypt | ||
| Abstract | ||
| Different regimens of superovulation were applied to 33 non-lactating Friesian cows for embryo production. These animals were randomly classifed into 6 groups of superovulatory treatments. Group 1 (n=5) was injected IM with a single dose of 2000 IU pregnant mare serum gonadotropins (PMSG) on day 10-12 of the estrous cycle. Luteolysis was induced by 2 injections of PGF2a (12 h interval) two days after PMSG treatment. The animal that showed signs of estrus was inseminated. Flushing was carried out non-surgically on day 7-9 of the estrus. Group 2 (n=5) and group 3 (n = 7) were treated as group 1, but the dose of PMSG was 2500 IU (Group 2) and 3000 10 (Group 3). The animals of Group 4 (n = 7) were primed with 200 IU/PMSG on day 5 and received 2800 IU/PMSG on day 10 of the cycle. All animals of this group were injected with 2 doses of PGF2a; 48 h after second dose of PMSG. Group 5 (n = 5) and 6 (n = 4) were treated as Group 4, but the priming doses of PMSG were 500 and 1000 IU/PMSG and the main doses of PMSG injected on day 10 were 2500 and 2000 IU/PMSG for Group 5 and 6, respectively. The mean values of CL were 7.6±1.36, 12.8±1.06, 9.57±1.17, 16.0±1.75, 13.8±0.96 and 5.57±0.85, for follicles, it was 1.8±0.66,2.6±0.40, 3.0±0.31, 4.57±0.81, 4.2-10.37 and 2.5±0.29, recovered embryos ova, it was 5.4±1.03, 8.6±0.60, 5.17±0.70, 7.07±1.06, 6.4±0.92 and 2.25 ± 0.85, for fertilized ova, it was 5.0±0.71, 7.0±0.54, 4.67±0.67, 6.5±0.85, 5.0±0.83 and 2.0±0.82, for transferable embryo, it was 4.2±0.79, 6.0±0.71, 3.8±0.54, 5.67±89, 4.4±0.67 and 1.75±0.85, for groups 1,2,3,4,5 and 6, respectively. The best results, as indicated by total ovarian response, CL, recovered embryo/ova, fertilized ova and transferable embryos were obtained in Group 4, when cows primed with 200 IU/PMSG early on Day 5 and 2800 IL•/PMSG on Day 10 of the estrous cycle. However, the finding results showed that, regimens of superovulatory treatment had a significant effect on the ovarian response and embryo recovery, and no significant effect on the embryo quality. Calving rates after transfer of single embryo were 44.1% and 42.9% for morula and blastocyst stages, respectively, with no significant differences between the 2 stages, and the overall mean was 43.6%, while, the calving rate after transfer double embryos was 47.6%. The grade cf embryos had a significant (P<0.06) effect on the calving rate, it was 53.57 and 33.33% for grade I and II embryos, respectively. | ||
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