P53 Codon 72 Gene Polymorphism in Patients with Hepatocellular Carcinoma on Top of Viral and Nonviral Etiologies | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 8, Volume 32, Issue 2, December 2012, Pages 101-114 PDF (301.14 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2012.35838 | ||
| Authors | ||
| Ayman Elsamanoudy* 1; Amal Selim1; Hussin Abdel Aziz1; Khaled Farid2; Tarek Besheer2 | ||
| 1Department of Medical Biochemistry, Faculty of Medicine, Mansoura University | ||
| 2Department of Tropical Medicine, Faculty of Medicine, Mansoura University | ||
| Abstract | ||
| Background and aim: Loss of p53 function has been suggested to be a critical step in multistage hepatocarcinogenesis. So, we aimed to investigate the frequency of P53 codon 72 gene polymorphism and its relation to plasma P53 levels in Egyptian patients with hepatocellular carcinoma (HCC) on top of viral and nonviral etiologies. Methods: This is a hospital-based case-control study which included 159 HCC patients in addition to 83 healthy volunteers as controls. Patients were classified into: 63 patients with HCC complicating cirrhosis due to HCV; 55 patients with HCC complicating cirrhosis due to HBV and 41 patients with HCC complicating cirrhosis due to nonviral causes. Quantitative determination of plasma P53 levels was performed by ELISA. P53 Arg 72 Pro gene polymorphism was carried out by conventional PCR followed by restriction enzyme digestion (PCR-RFLP). Results: There were significant increases in α.fetoprotein and plasma P53 levels in all studied groups in relation to the control group. AA genotype and A allele were more in the control group, PP genotype and P allele were more frequent in HCV related HCC group & HCC with non viral causes. AP genotype and P allele were more frequent in HBV related HCC group. P 53 plasma level showed significant increase in all groups in relation to the control group in AA genotype, AP genotype and PP genotype. There were significant increases in AP and PP genotype in all studied groups in comparison to AA genotype. Plasma P53 level showed significant increase in all groups in both allele A and allele P when compared with the control group. Also, it showed significant increase in their levels in P allele when compared with that of A allele in all studied groups. Conclusion: plasma p53 protein level could be considered as an additional tumor marker to AFP to increase the diagnostic potential of AFP in HCC patients. Therefore, P53codon 72 gene polymorphism could be used as an indicator of the genetic susceptibility for future development of HCC in Egyptian cirrhotic patients. | ||
| Keywords | ||
| P53 codon 72 polymorphism –HCC- plasma P53 | ||
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