Isolation Endophytic Bacteria from Leaf Glands and Seeds of Ardisia crenata Plant and its Molecular Detection | ||
| Egyptian Journal of Veterinary Sciences | ||
| Volume 56, Issue 6, June 2025, Pages 1265-1270 PDF (1.15 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2024.282284.2009 | ||
| Authors | ||
| jihan khalil khuder* 1; Amjad Abdul-Hadi Mohammed2 | ||
| 1department of biology, college of science ,university of mosul,mosul, country iraq | ||
| 2Biology department,college of science,Mosul university,musol city,iraq | ||
| Abstract | ||
| Isolation endophytic bacteria from leaf glands (nodules) and seeds of Ardisia crenata plant and cultured on Nutrient agar (NA) medium individually. The results of two types of bacterial isolated were grown in light yellow after incubation for 24 hours and took a circular shape slightly convex and Gram positive. The isolated bacteria from leaf nodules sensitive to all antibiotics used in this study, while the bacteria from seeds was resistance only to 10 µg/ml Cefotaxime and 10 µg/ml Ceftriaxone. The data of DNA sequencing analysis of the nitrogenous bases for 16S rRNA gene after PCR technique with the global database, National Center for Biotechnology Information (NCBI), showed 99.29% similarity between sequence of leaf isolated bacteria and Bacillus megaterium strain YSBM5 registered, therefore its recorded for the first times at Bacillus sp. JA Leaves, while 92.02% similarity between sequence of seeds isolated bacteria and Bacillus subtilis then its recorded at Bacillus sp. JA seed strain in NCBI. | ||
| Keywords | ||
| Ardisia crenata; Bacillus sp; 16S rRNA; PCR | ||
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