Mahmoud, F., Ma’moun, S., Farag, R., El Shafai, A., Abou zeid, A. (2019). Field Application of Honeybees’ Endogenous Lactic Acid Bacteria for the Control of American Foulbrood Disease. test, 11(1), 19-28. doi: 10.21608/eajbse.2019.36658
Fatma Mahmoud; Shireen A. M. Ma’moun; Rasha M. A. Farag; Akila M. El Shafai; Ahmed S. Abou zeid. "Field Application of Honeybees’ Endogenous Lactic Acid Bacteria for the Control of American Foulbrood Disease". test, 11, 1, 2019, 19-28. doi: 10.21608/eajbse.2019.36658
Mahmoud, F., Ma’moun, S., Farag, R., El Shafai, A., Abou zeid, A. (2019). 'Field Application of Honeybees’ Endogenous Lactic Acid Bacteria for the Control of American Foulbrood Disease', test, 11(1), pp. 19-28. doi: 10.21608/eajbse.2019.36658
Mahmoud, F., Ma’moun, S., Farag, R., El Shafai, A., Abou zeid, A. Field Application of Honeybees’ Endogenous Lactic Acid Bacteria for the Control of American Foulbrood Disease. test, 2019; 11(1): 19-28. doi: 10.21608/eajbse.2019.36658

Field Application of Honeybees’ Endogenous Lactic Acid Bacteria for the Control of American Foulbrood Disease

Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
Article 2, Volume 11, Issue 1, June 2019, Pages 19-28    PDF (1.06 M)
Document Type: Original Article
DOI: 10.21608/eajbse.2019.36658
Authors
Fatma Mahmoud1; Shireen A. M. Ma’moun1; Rasha M. A. Farag2; Akila M. El Shafai1; Ahmed S. Abou zeid1
1Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt
2Honeybee Research Department, Plant Protection Research Institute, Agriculture Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt
Abstract
           The purpose of this study is to investigate a new applicable and promising treatment for American Foulbrood (AFB) disease in honeybee colonies. AFB is a very serious honeybee disease caused by the spore-forming bacteria Paenibacillus larvae larvae (P. l. larvae). Five honeybee endogenous lactic acid bacteria (LAB), isolated from honeybee workers’ guts, were previously identified and tested for their inhibitory effects on P. l. larva in vitro. Artificial infection was accompanied by the administration of a mixture of the five LAB strains, two belong to Lactobacillus plantarum, two different strains of Lactobacillus kunkeei, one strain of Lactobacillus sp..  It was observed that the honeybee endogenous LABs inhibited P. l. larvae in an in vivo system. LAB mixture added to the larval food in honeybee colonies significantly reduced the number of infected larvae (P ≃ 0.000, p < 0.001). Confidence intervals analysis showed no significant difference from adding LAB to the food on first or second-day post infection and throughout the feeding period. In vivo studies demonstrated that LAB microbiota in Apis mellifera inhibits the bacterial brood pathogen P. l. larvae. The results pointed to new avenues for the prophylactic or therapeutic treatment of honeybee diseases.
Keywords
Apis mellifera; American foulbrood; lactic acid bacteria; Probiotic treatment; AFB control
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