Detection of Gastric Helicobacter like Organisms (GHLO) in Pet Animals by culture, ELISA and rapid urease test | ||
| Veterinary Medical Journal (Giza) | ||
| Volume 59, Issue 3, July 2011, Pages 355-362 PDF (1.27 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2011.368298 | ||
| Authors | ||
| El-S. Enany M* 1; Khafagy A1; Ibrahem H2 | ||
| 1Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Suez Canal University | ||
| 2Microbiology Department, Faculty of Medicine, Jazan University | ||
| Abstract | ||
| A total number of 50 stool, 50 saliva and 50 serum samples were randomly collected from 50 diseased dogs and cats of different breeds hosted in pet animals hospital in port- Said governorate. In addition 23 stomach samples were collected from domestic cats recently euthanized. Samples were prepared and plated on Brain heart infusion agar containing 5% horse serum and Dent supplement antibiotic and selective media. Colombia agar with 7% sheep blood and Dent supplement antibiotic. Each type of specimens. were subjected to isolation by culture and identification by biochemical activity and ELISA. The results demonstrate that among 50 studied dogs, GHLO isolates from stool and saliva samples were found to be 13 (26%) and 18 (36%) respectively and 31 out of 50 (62%) were +ve by ELISA. Also the results demonstrate that among 50 studied cats, GHLO isolates from stool and saliva samples were found to be 10 (20%) and 11 (22%) respectively and 25 out of 50 (50%) were +ve by ELISA. Selective 23 studied cat's stomachs, the results revealed that GHLO isolates were found to be 30.4% were +ve using culture and 47.8% and 52.2% were +ve using rapid urease test (RUT) and ELISA, respectively | ||
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