Hussein, A., Eid, A., Ibrahim, M., Lebdah, M. (2019). Molecular Analysis of Escherichia coli Strains Causing Respiratory Troubles in Chickens in Egypt. test, 47(2), 168-182. doi: 10.21608/zvjz.2019.10214.1026
Ashraf Hussein; Amal Eid; madeeha Ibrahim; Mohamed Lebdah. "Molecular Analysis of Escherichia coli Strains Causing Respiratory Troubles in Chickens in Egypt". test, 47, 2, 2019, 168-182. doi: 10.21608/zvjz.2019.10214.1026
Hussein, A., Eid, A., Ibrahim, M., Lebdah, M. (2019). 'Molecular Analysis of Escherichia coli Strains Causing Respiratory Troubles in Chickens in Egypt', test, 47(2), pp. 168-182. doi: 10.21608/zvjz.2019.10214.1026
Hussein, A., Eid, A., Ibrahim, M., Lebdah, M. Molecular Analysis of Escherichia coli Strains Causing Respiratory Troubles in Chickens in Egypt. test, 2019; 47(2): 168-182. doi: 10.21608/zvjz.2019.10214.1026

Molecular Analysis of Escherichia coli Strains Causing Respiratory Troubles in Chickens in Egypt

Zagazig Veterinary Journal
Article 5, Volume 47, Issue 2, June 2019, Pages 168-182    PDF (236.91 K)
Document Type: Original Article
DOI: 10.21608/zvjz.2019.10214.1026
Authors
Ashraf Hussein1; Amal Eid* 1; madeeha Ibrahim2; Mohamed Lebdah1
1Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Sharkia, Egypt
2Directorate of Veterinary Medicine, Zagazig, Sharkia, Egypt
Abstract
Colibacillosis is a complicated disease causing severe economic losses and challenging veterinarians and producers. Therefore, this study aimed to characterize avian pathogenic Escherichia coli (APEC) strains causing respiratory troubles in chickens. Thirty broiler chicken flocks (2013-2016 outbreaks) at age of 17-35 days from Sharkia, Ismailia, Dakahlia and Sinai showed respiratory troubles and greenish diarrhea. The postmortem findings revealed typical colisepticemia picture including air sacculitis, fibrinous pericarditis and perihepatitis. The percentage of APEC isolation was 100 % while 10 flocks suffered mixed infections. From 284 collected samples (air sacs, heart blood, lungs and liver), E. coli isolation was mostly from air sacs (76.1 %) and lung (73.2 %) followed by heart blood (67.6%) and liver (54.9%). Based on serogrouping, the most common serogroups were O78 and O2 with percentage of 15% each. Based on antimicrobial disc diffusion test, the lowest (32.7 %) and highest resistance (100 %) were to doxycycline versus lincomycin, spiramycin, oxacillin and amoxicillin. PCR analysis for 55 MDR E. coli isolates revealed 3 antibiotic resistance genes (blaTEM (87.3 %), blaCTX-M (85.5 %) and blaOXA (5.5 %)) and 6 virulence genes in two multiplex PCR (iucD (96 %), Fim H (92.7 %), iss (76.4 %), ompT (58.2 %), tsh (45.5 %) and cvaC (9 %). An association between virulence and multidrug resistance genes in E. coli was concluded, that hindered the control strategy. Therefore, alternative strategies were necessary to minimize the antibiotic use and reduce the virulent strains occurrence.
Keywords
Escherichia coli; Virulence; β-lactamase resistance; Broilers
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