Characterization and evaluation of probiotic strains isolated from different sources | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 20 January 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.344028.2389 | ||
| Authors | ||
| Khansa Mohammed Younis* ; Ashwaq Hazem Najem* | ||
| Department of Biology, College of Sciences, University of Mosul, Mosul, Iraq | ||
| Abstract | ||
| Background: A wide set of safe, industrially important microorganisms known as lactic acid bacteria are mostly utilized as probiotics and starting cultures, but they are also being explored as production systems in industrial biotechnology. The aim of this study was to isolate, identify and characterize lactic acid bacterial (LAB) isolates from dairy products and pickles samples, and evaluate their antimicrobial activity against some pathogenic organisms. Methods: Isolates were identified based on morphology, physiology, and biochemical tests. Further analysis of these isolates revealed that they were characterized based on their ability to grow at different pH, temperature, their ability to produce acids, and their tolerance to bile salts. The antibiotic resistance of all the isolates were determined following the method of Kirby Bauer disk diffusion assay. In vitro assessment of antimicrobial activity against pathogenic organisms was also tested. Morphological, physiological, and biochemical identification suggest that 16 of the isolates belong to lactic acid bacteria. The ability of the isolates to grow in different pH and temperatures, to produce acid, and to tolerate bile salts was observed. Results and Conclusion: The isolates were resistant to all antibiotics used. The two most active isolates selected showed their potential probiotic traits and demonstrated in vitro antibacterial activity against Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae. | ||
| Keywords | ||
| Probiotics; Lactic acid bacteria; Bile salts; Antimicrobial activity | ||
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