Antifungal Effect of Thymus vulgaris Methanolic Extract on Growth of Aflatoxins Producing Aspergillus parasiticus

Mossa, Monga I.

doi:10.21608/ejbo.2019.12540.1311

Antifungal Effect of Thymus vulgaris Methanolic Extract on Growth of Aflatoxins Producing Aspergillus parasiticus

Egyptian Journal of Botany

Article 2, Volume 61, Issue 2, August 2021, Pages 349-359 PDF (1.55 M)

Document Type: Regular issue (Original Article)

DOI: 10.21608/ejbo.2019.12540.1311

Author

Monga I. Mossa^*

Botany Department, Faculty of Science, Arish University, N. Sinai, Egypt

Abstract

THIS STUDY illustrates a simple method for the detection of the ability of Aspergillus parasiticus, isolated from peanut seeds, for the production of aflatoxins. The indicator of aflatoxins production ability is the appearance of colony reverse yellow color of the fungus on the agar media and the natural fluorescence of aflatoxin around the colony when observed under UV (black light 350BL) after three days of incubation at 28ºC. The morphological identification of A. parasiticus was confirmed by molecular identification rDNA sequencing. The addition of β-Cyclodextrin to media enhanced the natural fluorescence of aflatoxin around the colony under UV. The extract of Thymus vulgaris had an inhibitory effect on the growth of A. parasiticus. The minimum inhibitory concentration (MIC) of Thymus extract against A. parasiticus was 80μL of Thymus extract /10 ml of YSA medium. The effective concentration of Thymus extract causing a 50% inhibition of mycelial growth (EC50) was 20μL of Thymus extract /10mL of YSA medium. The chemical analysis of Thymus extract by Gas Chromatography-Mass Spectroscopy (GC-MS) illustrated that the thymol has a retention time (RT) (min) of 37.538 at the highest percent area (13.8717 Area %).

Keywords

Aflatoxin; Aspergillus parasiticus; Blue fluorescence; Extract; Thymus vulgaris

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	Antifungal Effect of Thymus vulgaris Methanolic Extract on Growth of Aflatoxins Producing Aspergillus parasiticus
Egyptian Journal of Botany
Article 2, Volume 61, Issue 2, August 2021, Pages 349-359 PDF (1.55 M)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2019.12540.1311
Author
Monga I. Mossa^*
Botany Department, Faculty of Science, Arish University, N. Sinai, Egypt
Abstract
THIS STUDY illustrates a simple method for the detection of the ability of Aspergillus parasiticus, isolated from peanut seeds, for the production of aflatoxins. The indicator of aflatoxins production ability is the appearance of colony reverse yellow color of the fungus on the agar media and the natural fluorescence of aflatoxin around the colony when observed under UV (black light 350BL) after three days of incubation at 28ºC. The morphological identification of A. parasiticus was confirmed by molecular identification rDNA sequencing. The addition of β-Cyclodextrin to media enhanced the natural fluorescence of aflatoxin around the colony under UV. The extract of Thymus vulgaris had an inhibitory effect on the growth of A. parasiticus. The minimum inhibitory concentration (MIC) of Thymus extract against A. parasiticus was 80μL of Thymus extract /10 ml of YSA medium. The effective concentration of Thymus extract causing a 50% inhibition of mycelial growth (EC50) was 20μL of Thymus extract /10mL of YSA medium. The chemical analysis of Thymus extract by Gas Chromatography-Mass Spectroscopy (GC-MS) illustrated that the thymol has a retention time (RT) (min) of 37.538 at the highest percent area (13.8717 Area %).
Keywords
Aflatoxin; Aspergillus parasiticus; Blue fluorescence; Extract; Thymus vulgaris

Statistics Article View: 833 PDF Download: 1,019