BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION | ||
| Research Journal of Applied Biotechnology | ||
| Article 9, Volume 3, Issue 1, June 2017, Pages 11-22 PDF (322.35 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/rjab.2017.57586 | ||
| Authors | ||
| Nashwa M. H. Rizk; Ayman S. Eldourghamy* ; Eman G. Elgamal | ||
| Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat city, Egypt | ||
| Abstract | ||
| Agricultural wastes are very rich in nutrients that can be used as complete balanced microbiological media for growing microbes in order to produce valuable commercial products. In this study, a biodegradation process of wheat bran and rice bran wastes was carried out to produce metalloprotease enzyme from B. thuringiensis 1257strain which is considered to be a hyper producer for that enzyme. Metalloprotease was partially purified by ammonium sulfate followed by application on Sephadex G-75 column. Gel filtration step resulted in more than 40 times fold purification of the purified enzyme. The enzyme activity was inhibited by EDTA (almost 80%) at 15 mM concentration. These agro-industrial wastes were used as substrates for economic production of the enzyme from B. thuringiensis compared with some bacterial isolates collected from different sites in Kafr El-Sheikh and Menofiya governorate, Egypt. Optimum temperature for enzyme activity was 40 °C. It also exhibited a broad pH activity range (6-9) with an optimum pH of 8. | ||
| Keywords | ||
| metalloprotease; B. thuringiensis1257; EDTA; agro-industrial waste; Sephadex G-75 | ||
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