Abd Allah, O., Eissa, I., Sherif, M., Dessouki, A., Elhalouse, A. (2015). Clinicopathological Studies on Blood of African catfish (Clarias gariepinus) Infected with Pseudomonas florescence. test, 20(1), 171-184. doi: 10.21608/scvmj.2015.65042
Osama Abd Allah; Ismaeil Eissa; Mona Sherif; Amina Dessouki; Abeer Elhalouse. "Clinicopathological Studies on Blood of African catfish (Clarias gariepinus) Infected with Pseudomonas florescence". test, 20, 1, 2015, 171-184. doi: 10.21608/scvmj.2015.65042
Abd Allah, O., Eissa, I., Sherif, M., Dessouki, A., Elhalouse, A. (2015). 'Clinicopathological Studies on Blood of African catfish (Clarias gariepinus) Infected with Pseudomonas florescence', test, 20(1), pp. 171-184. doi: 10.21608/scvmj.2015.65042
Abd Allah, O., Eissa, I., Sherif, M., Dessouki, A., Elhalouse, A. Clinicopathological Studies on Blood of African catfish (Clarias gariepinus) Infected with Pseudomonas florescence. test, 2015; 20(1): 171-184. doi: 10.21608/scvmj.2015.65042

Clinicopathological Studies on Blood of African catfish (Clarias gariepinus) Infected with Pseudomonas florescence

Suez Canal Veterinary Medical Journal. SCVMJ
Article 13, Volume 20, Issue 1, June 2015, Pages 171-184    PDF (1.6 M)
Document Type: Original Article
DOI: 10.21608/scvmj.2015.65042
Authors
Osama Abd Allah1; Ismaeil Eissa2; Mona Sherif3; Amina Dessouki4; Abeer Elhalouse* 3
1Clinical Pathology Dept., Faculty of Vet Medicine, Suez Canal Univ.
2Fish Diseases and Management Dept., Faculty of Vet Medicine, Suez Canal Univ.
3Animal Health Research Institute
4Pathology Dept., Faculty of Vet. Medicine, Suez Canal Univ., Ismailia, Egypt
Abstract
The present study was planned to investigate the clinicoplathological and histopathological changes on the blood of catfish (Clarias gariepinus) infected with Pseudomonas florescence. A total number of 240 catfish were collected from Ismailia channel and subdivided to 4 groups 60 for each: the control group, infected group, β-glucan group and β-glucan infected group. The blood samples were taken at 1, 3 and 5 weeks for hematological and biochemical examinations. Tissue samples from spleen, liver, kidney and gills were taken for histopathological techniques. The hematological results revealed significant decrease of RBCs count, Hb concentration and PCV% with significant increase of T.L.C, heterophils and lymphocytes of the infected group compared to the control group. The β-glucan groups showed significant increase of RBCs count, Hb and PCV% with significant increase of T.L.C, heterophils, lymphocytes and monocytes. Serum biochemical results showed marked elevation in ALT, AST, urea, creatinine, uric acid and glucose of the infected group. While, β-glucan improved the liver function, kidney function tests and glucose before and after the infection compared to the control group. The challenge test revealed a significant lower mortality percentage in the group received β-glucan supplemented diets. Histopathologically, marked degenerative changes and necrosis were evident in the infected group. While, β-glucan groups showed hyperactivity of melenomacrophage centers of haemopiotic organs. It could be concluded that β-glucan acts as a dietary supplement.
Keywords
Clinical pathology; Blood; Pseudomonas; African catfish
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