Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus | ||
| Journal of Applied Veterinary Sciences | ||
| Article 5, Volume 2, Issue 1, October 2017, Pages 35-42 PDF (314.06 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/javs.2017.65605 | ||
| Authors | ||
| Ehab E. Ibrahim* 1; Hamed A.H. El Helw2; Nermen Guda Shafik3; Manal Awad Mekhail4 | ||
| 1Department of Foot and Mouth Disease, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt | ||
| 2Veterinary Serum and Vaccine Research Institute, svri@idsc.gov.eg. | ||
| 3Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Egypt | ||
| 4Veterinary Serum and Vaccine Research Institute, Abbassia, Egypt | ||
| Abstract | ||
| Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 respectively and where 2.81; 2.81 and 2.8 respectively when measured by ELISA. Evaluation of Anti-FMD IgY conjugated with Horse radish peroxidase for detection of FMD virus in 100 tongue epithelium; 50 OP fluid samples; 20 nasal swabs from naturally infected cattle, and 100 tissue culture infected fluids revealed sensitivity of 85% indicating the success of preparation of anti-FMD immunoglobulins conjugated with horse radish peroxidase as local product available on request saving cost and time. | ||
| Keywords | ||
| ELISA; FMD; Immunization; PCR; Horse radish peroxidase; Serotypes | ||
| References | ||
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