PHYSIOLOGICAL STUDIES ON Digitalis lanata L. PLANT BY USING TISSUE CULTURE TECHNIQUE. | ||
| Journal of Plant Production | ||
| Article 9, Volume 3, Issue 2, February 2012, Pages 283-296 PDF (459.16 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2012.84054 | ||
| Authors | ||
| M. Nesem1; M. Khalil1; Kamilia T. Fouly2; S. Issa2 | ||
| 1Dept. Agric. Botany, Fac. Agric., Cairo University. | ||
| 2National Organization for Drug Control and Research. | ||
| Abstract | ||
| This experiment was carried out at Department of Biotechnology, tissue culture, green house and farm of Applied Research Center of Medicinal Plants (ARCMP) related to the National Organization for Drug Control and Research (NODCAR) throughout the period from 2005 to 2009 to study the effect of different concentrations of (IBA, NAA, BA and Kin) and their combinations on Digitalis lanata plant. This investigation included four major parts (starting stage, multiplication stage, callus induction stage and rooting stage) as well as Acclimatization stage. The data of this study revealed that in starting stage, MS medium supplemented with 3 mg/l IBA or with 2 mg/l Kin + 2 mg/l IBA gave the best results of survival percentage. The highest number of shoots obtained by explanted cultured on MS medium supplemented with 2 mg/l BA. The tallest shoots were obtained by cultured explant on MS medium supplemented with 2 mg/l Kin, 2 mg/l Kin + 2 mg/l IBA or 3 mg/l Kin + 3 mg/l IBA. MS medium supplemented with 2 mg/l IBA giving the highest number of leaves per explant. While, in multiplication stage, MS medium supplemented with 0.3 mg/l NAA + 0.5 mg/l BA gave the highest result of survival percentage. MS medium free hormones gave the tallest shoots, the highest amount of leaf number and root percentage. The highest number of shoots was obtained when cultured explant on MS medium supplemented with 0.4 mg/l IBA + 4.0 mg/l BA. Full MS salt strength medium supplemented with 0.4 mg/l NAA +4.0 mg/l Kin gave the highest survival percentage and leaf number. Full MS salt strength medium supplemented with 0.4 mg/l IBA +4.0 mg/l BA gave the longest shoots and the highest number of shoots. 3/4 MS salt strength medium supplemented with 0.4 mg/l IBA +4.0 mg/l Kin or 0.4 mg/l IBA +4.0 mg/l BA resulted in the highest root percentage. In callus induction stage, MS medium supplemented with 2 and 3 mg/l 2,4 D. with or without MnSO4 gave 100% survival percentage. The highest callus dry weight was obtained when cultured explant on MS medium supplemented with 4 mg/l 2,4 D. without MnSO4. In rooting stage, full MS salt strength medium supplemented with 0.5 mg/l IBA led to the highest number of roots, Full MS salt strength medium supplemented with 2 mg/l IBA gave the longest roots. Using peat moss as growing media produced the highest values of survival percentage, shoot length and number of leaves in acclimatization stage. | ||
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