desoky, S., EL Sayed, Y., Ibrahim, S. (2020). Toxicological Studies and Histopathological Changes on Black Bean Aphid, Aphis craccivora Induced by Entomopathogenic Fungi, Metarhizium anisopliae and Purpureocillium lilacinum. test, 12(1), 185-196. doi: 10.21608/eajbsf.2020.97626
Shimaa Mohamed desoky; Yasmein A. EL Sayed; Suzan A. Ibrahim. "Toxicological Studies and Histopathological Changes on Black Bean Aphid, Aphis craccivora Induced by Entomopathogenic Fungi, Metarhizium anisopliae and Purpureocillium lilacinum". test, 12, 1, 2020, 185-196. doi: 10.21608/eajbsf.2020.97626
desoky, S., EL Sayed, Y., Ibrahim, S. (2020). 'Toxicological Studies and Histopathological Changes on Black Bean Aphid, Aphis craccivora Induced by Entomopathogenic Fungi, Metarhizium anisopliae and Purpureocillium lilacinum', test, 12(1), pp. 185-196. doi: 10.21608/eajbsf.2020.97626
desoky, S., EL Sayed, Y., Ibrahim, S. Toxicological Studies and Histopathological Changes on Black Bean Aphid, Aphis craccivora Induced by Entomopathogenic Fungi, Metarhizium anisopliae and Purpureocillium lilacinum. test, 2020; 12(1): 185-196. doi: 10.21608/eajbsf.2020.97626

Toxicological Studies and Histopathological Changes on Black Bean Aphid, Aphis craccivora Induced by Entomopathogenic Fungi, Metarhizium anisopliae and Purpureocillium lilacinum

Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
Article 15, Volume 12, Issue 1, June 2020, Pages 185-196    PDF (1.17 M)
Document Type: Original Article
DOI: 10.21608/eajbsf.2020.97626
Authors
Shimaa Mohamed desoky1; Yasmein A. EL Sayed2; Suzan A. Ibrahim3
1Botany and Microbiology department, Faculty of Science, Suez University
2Plant Protection Research Institute Agriculture Research Center Dokki Giza Egypt*
3Plant Protection Research Institute Agriculture Research Center Dokki Giza Egypt
Abstract
Toxicological, histopathological and ultrastructure studies of Metarhizium anisopliae and Purpureocillium lilacinum infected Aphis craccivora nymph were investigated in this study. comparison between fungal spore suspensions and fungal culture filterates virulence effects of the two tested entomopathogenic fungi  against A. craccivora  nymph were done under laboratory condition and the toxicological bioassay results revealed that P.lilacinum has a high toxic effect than M. anisopliae in which the  LC50 of fungal spore suspensions and culture filterates after 7 days post-treatment was (7×102, 1×105 spore/ml and 3.2, 4.7 % ) for P. lilacinum and M.anisopliae, respectively. Treated nymph with spore suspensions was examined using both light and transmission electron microscope to study histopathological and ultrastructure changes compared with the control one. This comparison suggested that there is a deformation of the nymph body, complete destruction of all internal organs with an extensive invasion of the fungal spores throughout all internal body regions.
Keywords
Black aphid bean; Aphis craccivora; Biocontrol; Metarhizium anisopliae; Purpureocillium lilacinum; Histopathological; ultrastructure studies
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