(2007). A Broad-Specificity β-Glucosidase from a Wild Type of Yeast Isolate and Its Potential Use in Food Industry. test, 4(1), 63-72. doi: 10.21608/ajfs.2007.19633
. "A Broad-Specificity β-Glucosidase from a Wild Type of Yeast Isolate and Its Potential Use in Food Industry". test, 4, 1, 2007, 63-72. doi: 10.21608/ajfs.2007.19633
(2007). 'A Broad-Specificity β-Glucosidase from a Wild Type of Yeast Isolate and Its Potential Use in Food Industry', test, 4(1), pp. 63-72. doi: 10.21608/ajfs.2007.19633
A Broad-Specificity β-Glucosidase from a Wild Type of Yeast Isolate and Its Potential Use in Food Industry. test, 2007; 4(1): 63-72. doi: 10.21608/ajfs.2007.19633

A Broad-Specificity β-Glucosidase from a Wild Type of Yeast Isolate and Its Potential Use in Food Industry

Alexandria Journal of Food Science and Technology
Article 5, Volume 4, Issue 1, June 2007, Pages 63-72
Document Type: Original Article
DOI: 10.21608/ajfs.2007.19633
Abstract
A novel intracellular β-glucosidase was isolated from a wild type of yeast Trichosporon sp.). Production of enzyme
was stimulated with the highest level of production in medium containing 1 % lactose as a sole carbon source at
pH 7 and 30°C for 48 hr. The enzyme preparation exhibits activity towards several β-glucosidic substrates, indicating
that, it has broad- specificity, furthermore the activity towards lactose hydrolysis was the highest as compared with
other substrates. Also, it was able to catalyze transglycosylation. Therefore it was used for milk lactose hydrolysis as
well as oligosaccharides production. It has, thus potential use in food industry.
Keywords
β-glucosidase; lactose hydrolysis; transglycosylation
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